Moreover, organ shortage and the growing demand for organs prompt clinicians toexpand the donor pool by including extended criteria donors (ECD).8,9 In kidney transplantation, theuse of machine perfusion, in hypothermic or normothermic settings, has beneficialeffects in term of delayed graft function and primary nonfunction, for transplantfrom ECD and/or donation from cardiac death (DCD).10 These techniques to preserve and perfuse organs in hypothermic andnormothermic settings have been developed in liver, heart, and lung models also.
Leemkuil et al16 compared six hours of SCS (n = 10) and six hours ofHPP (n = 10) of human pancreases rejected for vascularizedorgan or islet transplantation. They had both DCD and donation after braindeath (DBD) pancreases. The HPP machine was a dual pulsatile perfusionsystem (Deltastream). The SP was 25 mmHg in all groups. The SCS and HPPsolution was UW and they added Acridin orange, in the solution, to evaluatethe perfusion. Concerning perfusion pressure, they reported no significantdifferences in flow between DCD and DBD pancreases. Concerning histologicalexamination, they reported no significant differences in terms of edemaformation, and acinar cell integrity loss between HPP and SCS in both DBDand DCD pancreases. Acridin orange staining was visible in all biopsies,confirming the uniform perfusion. Concerning immunohistochemicalexamination, after six hours of SCS, ATP concentration decreased in DCD andDBD pancreas, while, after six hours of HPP, ATP concentration increased inDCD and DBD pancreas. Concerning biochemical examination, amylase, lipase,and LDH increased after HPP and SCS. 1e1e36bf2d